Enter the primer sequence in the textbox for which the tm value needs to be calculated, and. This kit contains a sufficient supply of master mix and. This software can calculate the optimum pcr annealing temperature (tm value) of the primer.
TM Calculator with Cover 8 Digits x 20 pcs Lazada PH
Simply input your dna polymerase, primer.
To determine the optimal annealing temperatures for a given set of primers, use of the neb tm calculator is highly recommended.
To determine the optimal annealing temperatures for a given set of primers, use of the neb tm calculator is highly recommended. Amplification of a variety of human genomic amplicons. Select your dna polymerase, input your primer sequences, and provide your final primer. Tm calculator use this tool when designing pcr reaction protocols to help determine the optimal annealing temperature for your amplicon.
The neb tm calculator should be used to determine the annealing temperature when using this enzyme. Calculate tm and annealing temperatures of primer pairs using the neb tm calculator. Calculate the tm of primers and annealing temperature for different dna polymerases using this tool.
